README for Figure S5B.csv 
*** This file contains the raw data obtained on DNA and DNA-Rok ?75-96 complexes using bridging assay experiments represented in Figure S5B of 
Article: The B. subtilis Rok protein is an atypical H-NS-like protein irresponsive to physico-chemical cues
Authors: Erkelens, Qin, van Erp, Keek, Markus and Dame 
Journal:  
DOI:
Corresponding author: rtdame@chem.leidenuniv.nl


Legend Figure S5: Rok ?75-96 compacts and bridges DNA but with decreased cooperativity. A)  Histograms of Root 
Mean Square displacement (RMS) obtained for 32%GC DNA as a function of Rok ?75-96 at concentrations of 0, 1, 2, 3, 
4, 5, 7, 8, 9, 10, 11, 12, 13, 14 and 15 nM as measured by TPM in the presence of 50 mM KCl. The histograms were 
fitted to Gaussian distributions, in which the RMS value at ~150 nm represents bare DNA and the population with an 
RMS at ~100 nm represents DNA bound by Rok ?75-96. The bare DNA and Rok-DNA complex populations are highlighted 
with a light blue and magenta box, respectively. The data for each concentration originates from at least two 
independent measurements. B) DNA recovery (%) as a function of protein concentration in M in the presence of 50 
mM KCl at 25C. For reference, Rok WT is shown (reproduced from figure 2A). Data are plotted as mean values and 
the error bars represent the standard deviation from three independent measurements. Dashed lines are lines to 
guide the eye.  

*** The data were obtained using bridging assay experiments as described in the associated article. 

***The data labeled overview represents the values plotted in the graph of figure S5B. The raw data contains the individual replicates.
Column A: Sample number
Column B: Presence of bait DNA in sample
Column C: Presence of 32P-labeled prey DNA in sample
Column D: Amount of Rok ?75-96 in the sample in micromolar
Column E: Counts per minutes 
Column F: DNA recovery (%)

Columns B, C and D indicate the contents of the sample. Column E indicates the radioactivity of the sample. 
Column F indicates the DNA recovery, which is calculated by subtracting the value in column E of the control 
(sample minus bait DNA) from the sample itself. This value is then divided by the values in column E of sample 1 
and multiplied by 100%. 









